She performed the classic album's final song Twist And Shout, accompanied by a full choir of backing singers.
She told the BBC: "It's a huge, raucous song which everybody knows."
Other musicians who joined the recording at Abbey Road studios included Mick Hucknall and Joss Stone.
The Fab Four recorded almost all of the album in a day-long recording session 50 years ago, on 11 February 1963.
By the end of the long day, Lennon - who had struggled with a cold - performed what is the now legendary cover version of the Isley Brother's track Twist And Shout.
Knight said: "You can hear after the first verse that he's done, he's spent but he's still giving it.
"I heard he ripped his shirt off cause he was so hot and was getting down and dirty and that's what you want, you can hear he's giving every last drop."
Stereophonics singer Kelly Jones - who sang I Saw Her Standing there - said the album "was the sound of a band capturing their raw energy".
Earlier in the day, he had busked outside the studio with Radio 2 Breakfast presenter Chris Evans.
The tracks were broadcast live on Radio 2 over 12 hours.
Soul singer Joss stone performed The Beatles' cover of A Taste of Honey, taken from the hit film.
Backed by a full string section, the 25-year-old took the lead in a jazzy version of the song.
She described her self as an "idiot" as she "wasn't aware of the song actually, which is pretty bad".
But she added: "I'm glad it turned out alright."
Girl group sound
Blur guitarist Graham Coxon performed the less well known track Baby It's You, written by Burt Bacharach, and stripped it back to the sound of the original version by girl group The Shirelles.
He said: "I'm quite pleased to be doing that because it's not one that people immediately think about a Beatles song, so it's a sort of a soul song, which has its own challenges.
"Although I'm not a soul singer, maybe I should have gargled some rice crispies to get a rough voice going, cause I'm not very gravelly."
Ian Broudie of the Lightning Seeds covered Do You Want To Know A Secret - which originally carried vocals from George Harrison - at the Radio 2 session.
He said that while The Beatles' undoubtedly changed music, their debut was more of "a charming start".
"It was basically their live set. This is how they would have sounded at a live gig before they signed with George Martin. It's a great photo."
Of the original recording, Mark Lewisohn, in his book The Complete Beatles Chronicle, wrote: "There can scarcely have been 585 more productive minutes in the history of recorded music."
Broadcaster Stuart Maconie, who is hosting a BBC Four documentary about the Radio 2 recording, said it was hard to imagine the original sessions.
"I got here this morning and what struck me was that there are so many people here - bands, crews, journalists - and how different it must have been that morning 50 years ago when the Beatles arrived in their van.
"I'm fascinated whether they knew they were changing the history of the world. Did they have an inkling? I wonder if McCartney knew?"